Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae.
نویسندگان
چکیده
Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20M. pneumoniae genomes per reaction.
منابع مشابه
Evaluation of five real - time PCR assays for detection of Mycoplasma pneumoniae 7 8 Running title : Real - time PCR for detection of
25 Four commercial real-time PCR assays to detect M. pneumoniae were tested and the results 26 compared with an in-house approach. Despite differences of crossing threshold values up to 4, 27 assays were able to detect at least 20 cfu/5 μl (52 fg DNA/5 μl) of sample with best clinical 28 sensitivity of Diagenode kit. 29
متن کاملEvaluation of five real-time PCR assays for detection of Mycoplasma pneumoniae.
Four commercial real-time PCR assays to detect Mycoplasma pneumoniae were tested, and the results were compared with the results for an in-house approach. Despite differences of crossing threshold values of up to 4, assays were able to detect at least 20 CFU/5 μl (52 fg DNA/5 μl) of sample with the Diagenode kit showing the best clinical sensitivity.
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ورودعنوان ژورنال:
- Diagnostic microbiology and infectious disease
دوره 88 2 شماره
صفحات -
تاریخ انتشار 2017